Abstract:This paper used the rare edible fungi Fistulln hepatica as raw material to establish the enzymatic hydrolysis technology for its polypeptides by single factor test and response surface methodology.Taking the degree of hydrolysis as the index，this paper used five kinds of proteinases （acid protease，alkaline protease，trypsin，pepsin，and papain）to hydrolyze F.hepatica.The effects of different reaction time，substrate concentration，pH value，temperature，and enzyme amount on the degree of hydrolysis in the process of extracting polypeptide from F.hepatica were investigated，and the technological conditions were optimized by response surface methodology.The results showed that trypsin was the most suitable protease to hydrolyze F.hepatica polypeptides.When hydrolysis time was 5 h，substrate concentration was 5%，pH value was 8，enzyme addition was 10 000 U/g，and hydrolysis temperature was 45 ℃，the hydrolysis degree of F.hepatica polypeptides was 59.17% and the protein content reached 33.67%.