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投稿时间：2022-12-30
投稿时间：2022-12-30
中文摘要: 以番石榴叶为原料，通过单因素结合正交试验确定超声波辅助低共熔溶剂（deep eutectic solvents，DES）提取工艺，以NaNO2-Al（NO3）3 比色法结合高效液相色谱（high performance liquid chromatography，HPLC）法测定分析不同品种叶片总黄酮与主要黄酮类成分含量差异。结果表明，各因素对番石榴叶总黄酮提取率的影响程度为DES 含水率＞DES 组成＞料液比。最佳提取工艺：以含水率为25%的氯化胆碱与乙二醇组成的DES 为溶剂，料液比1∶30（g/mL），总黄酮平均提取率为14.95%。‘全红’总黄酮含量226.62 mg/g，高于其余品种（45.12～183.56 mg/g），且总黄酮在‘全红’‘珍珠’‘新世纪’中分布为嫩芽＜嫩叶＜老叶。‘全红’中4 种主要黄酮类成分含量为21.03 mg/L，番石榴苷、萹蓄苷、瑞诺苷和槲皮素，含量分别为7.25、7.94、3.96 mg/L 和1.88 mg/L。综合总黄酮和4 种黄酮类化合物含量，‘全红’是最适于番石榴叶黄酮开发的原料。
Abstract:The ultrasonic-assisted deep eutectic solvents（DES）extraction process was determined based on Guava leaves through a single-factor experiment combined with orthogonal experimental design.The total flavonoid and major flavonoid content differences in different cultivars of leaves were determined using the NaNO2-Al（NO3）3 colorimetric method combined with high-performance liquid chromatography （HPLC）.The results showed that the factors affecting the extraction rate of total flavonoids from Guava leaves were in the sequence of DES water content＞DES composition＞liquid-to-material ratio.The optimal extraction should be performed using DES combined with choline chloride and ethylene glycol（25%water content）as the extraction solvents with a liquid-to-material ratio of 1∶30（g/mL），resulting in an average extraction rate of total flavonoids of 14.95%.The total flavonoid content of'Quanhong'was 226.62 mg/g，higher than other cultivars（45.12 mg/g to 183.56 mg/g），and the distribution of total flavonoids in'Quanhong'，'Zhenzhu'，and'Xinshiji'was tender shoots＜tender leaves＜old leaves.The content of four major flavonoid compounds in 'Quanhong' was 21.03 mg/L，including guaijaverin，avicularin，reynoutrin，and quercetin，with content of 7.25 mg/L，7.94 mg/L，3.96 mg/L，and 1.88 mg/L，respectively.Considering the content of both total flavonoids and the four major flavonoid compounds，'Quanhong'was the most suitable raw material for the development of Guava leaves flavonoid.
keywords: Guava leaves deep eutectic solvents ultrasonic-assisted extraction flavonoids cultivar differences
文章编号：202314022 中图分类号： 文献标志码：
基金项目:广西农业科学院科技发展基金项目（桂农科2022JM92）；广西农业科学院基本科研业务专项项目（桂农科2021YT144、桂农科2021YT146）
| 作者 | 单位 |
| 罗朝丹1，2，任二芳1，2，黄燕婷1，2，李建强1，2*，苏艳兰1，2，冯春梅1，2，罗小杰1，2，程三红1，2 | 1.广西壮族自治区亚热带作物研究所，广西 南宁 530001；2.广西亚热带水果加工工程技术研究中心，广西 南宁 530001 |
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