Abstract:In this study，copper sulfide nanoparticles doped with 4-mercaptoboric acid（CuS@MPBA NPs）exhibiting near-infrared laser response and high photothermal conversion properties were synthesized for the detection and inactivation of foodborne pathogens like Escherichia coli（E.coli）and Staphylococcus aureus（S.aureus）.When CuS@MPBA-NPs were combined with the above pathogenic bacteria，a complex was formed through the interaction of the 4-mercaptophenylboric acid with cis-diol of saccharides on the bacterial cell.The complex was separated from non-adsorbed CuS@MPBA-NPs through centrifugation and collected as a precipitate.Laser irradiation of the precipitate complex raised the temperature of the solution and enabled temperature-sensitive detection of bacteria in the range of 10 CFU/mL-107 CFU/mL for both E. coli and S. aureus. During the temperature-sensitive detection process，bacteria could be inactivated simultaneously due to the increase in temperature，providing antibacterial efficiency of up to 98.3% and 98.7% against E.coli and S.aureus，respectively.