Abstract:Thermo-sensitive luteolin-Zn（Ⅱ）complex molecularly imprinted polymers（TMIPs）were synthesized for the separation of C-glycosylflavones from Indocalamus tessellates leaves with luteolin-Zn（Ⅱ）complex as the template molecule，acrylamide as functional monomer，N-isopropylacrylamide as thermo-sensitive monomer，and N，N＇-methylenebisacrylamide as cross-linker.The results showed that the optimal preparation condition of TMIPs was template molecule: functional monomer：thermo-sensitive monomer: cross-linker=1∶4∶16∶30（mole ratio）.The lower critical solution temperature of TMIPs was about 32 ℃.The adsorption performance was consistent with the Langmuir adsorption model and the pseudo-first-order kinetic model.The adsorption capacity of TMIPs for luteolin reached 36.06 μmol/g，and its imprinting factor was 3.18.Compared with apigenin and rutin，the selectivity coefficients for luteolin reached 1.83 and 2.18，respectively.TMIPs were sensitive to temperature and easy to control adsorption and desorption.TMIPs were applied for the separation of C-glycosylflavones from I. tessellates leaves，with adsorption capacity for vitexin，isovitexin，orientin，and isoorientin reaching 32.99，27.73，35.16，and 36.05 μmol/g，respectively.