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投稿时间：2022-10-05
投稿时间：2022-10-05
中文摘要: 以香菇为试材，采用单因素试验结合正交试验优化香菇多糖的提取工艺，研究香菇多糖的抗氧化活性。该试验采用酶解辅助法提取香菇多糖，考察纤维素酶添加量、提取温度、提取时间、料液比对香菇多糖含量的影响。结果表明：香菇多糖的最佳工艺条件参数为纤维素酶添加量0.4%、提取温度60 ℃、提取时间2 h、料液比1∶20（g/mL）。在该工艺条件下，香菇多糖含量达到5.73%，相对标准偏差（relative standard deviation，RSD）为1.73%，表明该工艺稳定可行。在最佳工艺条件下得到的香菇多糖提取物的羟基自由基清除率达到31.34%，香菇多糖具有较强的体外抗氧化活性。
Abstract:The single factor test and orthogonal design were employed to optimize the enzymatic hydrolysis-assisted extraction of polysaccharide from Lentinus edodes，and the antioxidant activity of the obtained polysaccharide was studied.The effects of enzyme addition，extraction temperature，extraction time，and solid-to-liquid ratio on the polysaccharide yield were investigated.The results showed that the optimum conditions were extraction with the solid-to-liquid ratio of 1∶20（g/mL）and 0.4% cellulase at 60 ℃for 2 h. Under the optimum extraction conditions，the yield of polysaccharide reached 5.73%（RSD=1.73%），which indicated that the extraction process was stable and feasible.The polysaccharide extracted under the optimum conditions showed the hydroxyl radical scavenging rate of 31.34% and had strong antioxidant activity in vitro.
keywords: Lentinus edodes polysaccharide enzymatic hydrolysis-assisted method process optimization orthogonal test antioxidant activity
文章编号：202312016 中图分类号： 文献标志码：
基金项目:天津市科技计划项目（20ZYCGSN00150）
| 作者 | 单位 |
| 赵茹1，康明丽1*，张琴1，张扬2，朱志强2，3，周永斌2，3 | 1.河北科技大学食品与生物学院，河北石家庄 050000；2.国家农产品保鲜工程技术研究中心（天津），农业农村部农产品贮藏保鲜重点实验室，天津 300384；3.天津市农业科学院农产品保鲜与加工技术研究所，天津 300384 |
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