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投稿时间:2022-10-08
投稿时间:2022-10-08
中文摘要: 以银杏为原料,采用正交试验设计优化复合酶处理对银杏内酯B 和总酚含量的影响。在单因素试验的基础上,采用L9(34)正交试验设计,考察α-淀粉酶添加量、糖化酶添加量、酶解温度、酶解时间4 个因素对银杏内酯B 和总酚含量的影响。结果表明:在α-淀粉酶添加量为6 U/mL、糖化酶添加量为9 U/mL、酶解温度为60 ℃、酶解时间为2 h的条件下,银杏内酯B 和总酚含量最高,银杏内酯B 含量为(49.29±0.87)μg/mL,总酚含量为(455.18±4.05)μg/mL,分别是酶解前的(1.82±0.02)倍和(1.32±0.02)倍,明显提高了目标产物的含量。
Abstract:The effects of complex enzyme treatment on the content of ginkgolide B and total phenols from ginkgo were analyzed and optimized by the orthogonal design.On the basis of single factor experiment,L9(34)orthogonal experimental design was used to investigate the effects of α-amylase additive amount,glycosylase additive amount,enzymatic hydrolysis temperature,and enzymatic hydrolysis time on the content of ginkgolide B and total phenols.The results presented the following optimized parameters:6 U/mL α-amylase,9 U/mL glycosylase,enzymatic hydrolysis temperature of 60 ℃,and enzymatic hydrolysis time of 2 h. Under the optimized conditions,the content of ginkgolide B and total phenols reached(49.29±0.87)μg/mL and(455.18±4.05)μg/mL,respectively,which were(1.82±0.02)times and(1.32±0.02)times those before enzymatic hydrolysis,significantly increasing the content of the target product.
文章编号:202312003 中图分类号: 文献标志码:
基金项目:江苏省重点研发计划(现代农业)(BE2022367)
作者 | 单位 |
杨计林1,2,张瑜2,朱勇生2,李思媛2,邸清茹2,端木传宇1,2,刘小莉1,2* | 1.江苏大学食品与生物工程学院,江苏镇江 212013;2.江苏省农业科学院农产品加工研究所,江苏南京 210014 |
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