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投稿时间：2022-12-08
投稿时间：2022-12-08
中文摘要: 为提高金耳酵母状孢子多糖得率，在单因素试验的基础上，通过Plackett-Burman试验和正交试验优化金耳酵母状孢子多糖的提取工艺；并通过测定金耳酵母状孢子多糖DPPH自由基和羟自由基清除率对其体外抗氧化活性进行探究。结果表明，金耳酵母状孢子多糖最佳提取工艺条件：纤维素酶200 U/g孢子粉，果胶酶50 U/g孢子粉，酶解时间80 min，酶解温度55℃，酶解pH值为4.5，料液比1∶40（g/mL），在该提取条件下，金耳酵母状孢子多糖得率为（8.41±0.36）% 。体外抗氧化试验表明，金耳酵母状孢子多糖对DPPH自由基和羟自由基的IC50值分别为7.06 mg/mL和19.33 mg/mL。
Abstract:The polysaccharide was extracted from the yeast-like conidia of Naematelia aurantialba.The extraction process was optimized by the Plackett-Burman design and orthogonal test on the basis of single factor tests.The antioxidant capacity of polysaccharide in vitro was evaluated based on DPPH and hydroxyl radical scavenging activities.The results showed that the optimum extraction conditions were cellulase addition of 200 U/g of yeast-like conidia powder，pectinase addition of 50 U/g of yeast-like conidia powder，and enzymolysis at 55℃，pH4.5，and solid-to-liquid ratio of 1∶40（g/mL）for 80 min.Under these conditions，the yield of polysaccharide was（8.41±0.36）% .The IC50values of the extracted polysaccharide for DPPH and hydroxyl radical scavenging were 7.06 mg/mL and 19.33 mg/mL，respectively.
keywords: yeast-like conidia of Naematelia aurantialba polysaccharides dual-enzymatic orthogonal test antioxidant
文章编号：202311019 中图分类号： 文献标志码：
基金项目:山东省现代农业产业技术体系食用菌产业体系烟台综合试验站（SDAIT-07-14）；中国农业大学烟台研究院校级课题（U2021008）
| Author Name | Affiliation |
| LIU Zi-qi，LI Zhong-qi，DING Qiang，WANG Hong-lei* | Yantai Institute of China Agricultural University，Yantai 264670，Shandong，China |
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