Abstract:The enzymatic hydrolysis process of raspberry pulp polysaccharide was optimized by response surface methodology，and then the anti-fatigue activity of the polysaccharide was studied.Plackett-Burman design was employed to screen out the main factors affecting the yield of raspberry polysaccharide，which were enzymatic hydrolysis temperature，enzymatic hydrolysis time，and enzyme dosage.The enzymatic hydrolysis conditions of polysaccharides from raspberry were optimized by response surface methodology.The optimum conditions were material-to-liquid ratio of 1∶20（g/mL），pH5，enzyme dosage of 0.85% ，and enzymatic hydrolysis at 44℃for 1.4 h.Under the optimal conditions，the yield of polysaccharides extracted from raspberry by hydrolysis with cellulase was 2.73% .The results of weight-bearing test with mice showed that the raspberry pulp polysaccharide obtained by hydrolysis with cellulase prolonged the time of weight-bearing swimming，improved the exercise endurance，significantly elevated the levels of liver glycogen and muscle glycogen，and lowered the serum levels of urea nitrogen and lactic acid in mice.The raspberry pulp polysaccharide had good anti-fatigue effect.