Abstract:To clarify the differences in the nutrients and volatile components of Boletus caps and stalks from Xiangcheng in Sichuan and Chuxiong in Yunnan，this study determined the content of moisture，protein，crude fat，polysaccharides，and amino acids，and determined the volatile components using headspace solid-phase microextraction combined with gas chromatography-mass spectrometry.The results showed that there were significant differences in the nutrient content of Boletus from different origins.Among them，the protein content of the Boletus cap in Chuxiong was the highest [（33.52±0.76）%]，the crude fat content of the Boletus stalk in Xiangcheng was the highest[（5.46±0.13）%]，and polysaccharide content of Boletus cap in Chuxiong was the highest[（19.22±0.09）%].The total amount of amino acids of Boletus stalk in Xiangcheng was the highest，and the cap amino acid score of Boletus in Xiangcheng was higher than that of the other three types of Boletus.Forty-five volatile compounds were identified，including five hydrocarbons，22 aldehydes，seven alcohols，eight ketones，and three nitrogen-containing sulfur and heterocycles.The analysis of the main volatile aroma components of Boletus by relative odor activity value（ROAV）method indicated that the key volatile aroma compounds of Boletus in Xiangcheng were 1-octene-3-one and 1-octene-3-alcohol，while the key volatile aroma compound of Boletus in Chuxiong was only 1-octene-3-one.There were great differences in the composition and content of flavor substances between Boletus in Chuxiong and Xiangcheng，and the content of nutrients was also different.The nutrients and flavor quality of Boletus stalk in Xiangcheng were optimal.