Abstract:Conjugated linoleic acid（CLA）is a general term for a class of functional fatty acids with anti-cancer，anti-atherosclerosis，lipid-lowering，and various other physiological activities.It has great application potential in the fields of food，medicine and animal husbandry.Due to limited natural sources，CLA is mainly synthesized by chemical and biological methods.CLA by biological synthesis has single isomers with high safety，which is the optimal choice for CLA synthesis.Among the biological methods，enzymatic synthesis of CLA is currently a research hotspot for the high purity and high content of products and high synthesis efficiency.In this study，the enzymatic synthesis pathway of CLA and the key enzymes in the pathway were reviewed，to provide guidance for improving the efficiency of CLA biosynthesis.