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投稿时间：2022-02-14
投稿时间：2022-02-14
中文摘要: 该文建立高效液相色谱（high performance liquid chromatography，HPLC）法测定饮料中甜菊醇的定量分析方法，并优化饮料中甜菊糖苷水解成甜菊醇的条件。采用3 mmol/g路易斯酸水解，无水乙醇提取，以70%乙腈水溶液（体积分数）作为流动相，使用ZORBAX SB-C18色谱柱。结果表明，甜菊醇在5.0 μg/mL～100.0 μg/mL的浓度范围内线性关系较好（r2≥0.999），平均回收率在81.9%～92.3%，相对标准偏差在1.17%～3.67%。饮料中甜菊糖苷转化甜菊醇的最佳工艺条件：催化剂为氯化铁，提取温度100℃，甜菊糖苷浓度300 mg/mL，提取时间3 h，催化剂含量3 mmol/g。
Abstract:A high performance liquid chromatography （HPLC）method was developed for the determination of steviol in beverage，and the conditions for the hydrolysis of stevioside into steviol were optimized.Specifically，the samples were hydrolyzed by 3 mmol/g Lewis acid and then extracted by 100%ethanol.HPLC was performed with ZORBAX SB-C18 column and 70%（volume fraction）acetonitrile aqueous solution as the mobile phase.The result suggested that the steviol had good linear relationship （r2≥0.999）within the concentration range of 5.0 μg/mL-100.0 μg/mL.The average recovery ranged from 81.9%to 92.3%，and the RSD from 1.17%to 3.67%.The hydrolysis conditions were optimized as hydrolysis with 3 mmol/g ferric chloride as the catalyst and the stevioside concentration of 300 mg/mL at 100℃for 3 h.
keywords: Lewis acid stevioside steviol high performance liquid chromatography（HPLC） acid hydrolysis
文章编号：202308026 中图分类号： 文献标志码：
基金项目:天津市市场和质量监督管理委员会科技计划项目（2018-W05）
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