Abstract:A polymerase chain reaction（PCR）visualization detection method was established using G-quadruplex and thioflavin T as the reporter system.A common gene amplification instrument and a blue light were the only items of equipment used in the entire process.After the reaction，the results were directly assessed by observing the fluorescence with the naked eye，without opening the lid of the reaction tube，which avoided PCR product contamination.Specific primers were designed to target pig-derived DNA，and a visual detection method for pig-derived meat was established.By comparing commercially available beef，sheep，rabbit，chicken，and duck meat，the specificity of the detection method was found to excellent，and the detection limit reached 0.01 ng/μL.We were able to detect 1%pork in DNA from mixed meat samples，and the method was successfully applied to the detection of pork in actual food samples.Compared with the commonly used real-time fluorescent PCR method，our method not only shortened the detection time，but also did not require the use of an expensive fluorescent PCR instrument and did not require a probe modified with a fluorophore.Thus，this method is suitable for the rapid detection of pig-derived meat under simple conditions.