Abstract:Using Cordyceps militaris fruiting body as raw material，this paper used response surface methodology（RSM）to optimize the extraction process of C.militaris polyphenols and analyzed the antioxidant activity of C.militaris polyphenols.Based on single factor test，using yield of polyphenols as index，this paper used Box-Behnken design to conduct RSM and determine the optimal extraction process of C.militaris polyphenols.The total antioxidant capacity，DPPH free radical scavenging ability，and hydroxyl free radical scavenging ability of polyphenols in vitro were analyzed.The results showed that the optimal extraction process of C.militaris polyphenols was 51 ℃ of extraction temperature，1.6 h of extraction time，and 49∶1（mL/g）of liquid-solid ratio，and the yield of polyphenols was 6.03 mg/g under optimized conditions.The results of antioxidant activity analysis showed that the total antioxidant capacity of C.militaris polyphenols increased with the increase in mass concentration in the range of 0-0.4 mg/mL，and was always higher than that of vitamin C solution in the same concentration.The semi-inhibitory mass concentration of DPPH free radical in polyphenol solution was 19.52 μg/mL，which was 75.25%of that in vitamin C solution，and the semi-inhibitory mass concentration of hydroxyl radical was 86.47μg/mL，which was 8.31%of that in vitamin C solution.The extraction process of C.militaris polyphenols is feasible，and C.militaris polyphenols has relatively strong antioxidant activity.