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投稿时间：2022-01-10
投稿时间：2022-01-10
中文摘要: 以宁夏楼葱假茎部位为材料，采用酶辅助有机溶剂萃取法，通过正交试验优化楼葱中脂溶性物质的提取条件，并利用滤纸片法测定提取物对枸杞源链格孢霉菌的抑菌作用。结果表明：以乙酸乙酯作为萃取液时，楼葱脂溶性物质最佳提取条件为料液比1 ∶40（g/mL）、加酶量0.3%、酶解时间60 min、萃取时间36 h，该条件下楼葱脂溶性物质得率为（6.33±0.24）%。抑菌试验结果表明：在放置含提取物滤纸片的链格孢霉菌培养基中，抑菌圈直径为（19.58±0.31）mm，最小抑菌浓度为1.250 mg/mL，表明楼葱脂溶性提取物对枸杞源链格孢霉菌有较好的抑制作用。
Abstract:The stalk of Allium fisulosum in Ningxia were extracted by the enzyme-assisted organic solvent extraction method，and the extraction conditions of liposoluble components in A. fisulosum were optimized by the orthogonal experiment. The antibacterial effect on the postharvest Alternaria alternata of Lycium barbarum was determined by the filtering paper method.The results showed that when ethyl acetate was used as the extract liquid，the optimal extraction conditions for the liposoluble components of A. fisulosum were the solid-liquid ratio of 1 ∶40（g/mL），enzyme addition of 0.3%，enzymolysis for 60 min and extraction for 36 h. Under these conditions，the yield of the liposoluble components of A.fisulosumwas（6.33±0.24）%.The results of the antibacterial test showed that the diameter of the inhibition zone was（19.58±0.31）mm and the minimum inhibitory concentration was 1.250 mg/mL on the A. alternata culture medium containing the extract filtering paper. It showed that the liposoluble component extract of A.fisulosum had a certain inhibitory effect on A.alternata from L.barbarum.
keywords: Allium fisulosum liposoluble component extract orthogonal test Alternaria alternate antibacterial effect
文章编号：202307016 中图分类号： 文献标志码：
基金项目:宁夏回族自治区青年拔尖人才培养工程项目（2021JCTJ0030）；宁夏食品微生物应用技术与安全控制重点实验室平台建设项目（2019YDDF0062）
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