丹凤牡丹花蕊水溶性多糖纯化、理化性质及抗氧化活性

段雪莹; 罗磊*; 李瀚姝; 赵一帆; 马潇

###

DOI:10.12161/j.issn.1005-6521.2023.07.012

食品研究与开发:2023,44(7):83-89

查看/发表评论过刊浏览高级检索 HTML PDF

←前一篇 | 后一篇→

码上扫一扫！

丹凤牡丹花蕊水溶性多糖纯化、理化性质及抗氧化活性

段雪莹，罗磊*，李瀚姝，赵一帆，马潇

(河南科技大学食品与生物工程学院，河南洛阳 471000)

Purification，Physicochemical Properties and Physiological Activity of Water-Soluble Polysaccharides from Stamens of Paeonia suffruticosa

DUAN Xue-ying，LUO Lei*，LI Han-shu，ZHAO Yi-fan，MA Xiao

(The crude polysaccharides were extracted from stamens of Paeonia suffruticosa by ultrasonic complex enzyme and obtained by decolorization and proteinization. Three water -soluble polysaccharide（WSP）components（WSP-1，WSP-2 and WSP-3）were purified by DEAE-52 cellulose column chromatography，and were further purified by Sephadex G-100 gel chromatography with high yield and good hypoglycemic activity.The results showed that WSP-3 was an acidic homozygous polysaccharide with a molecular weight of 14 351 Da，and the residues contained α-glycosidic bonds with a triple helix structure. WSP-3 was observed by scanning electron microscope as a rod of uneven size. Antioxidant experiments showed that WSP-3 had certain scavenging ability on DPPH·，·OH，and ABTS+·，and the IC50 was 38，3.05 and 39 μg/mL，respectively.)

摘要

图/表

参考文献

相似文献

本文已被：浏览 475次下载 303次
投稿时间：2022-02-27

中文摘要: 以丹凤牡丹花蕊为原料，采用超声波复合酶法提取，经过去色、去蛋白得到粗多糖。采用DEAE-52 纤维素柱层析纯化得到3 个水溶性多糖（water-soluble polysaccharide，WSP）组分（WSP-1、WSP-2、WSP-3），并采用葡聚糖凝胶G-100 凝胶色谱法对得率高且降糖作用好的多糖组分进一步纯化。结果表明：WSP-3 是分子质量为14 351 Da 的酸性均一多糖，残基同时包含了α 构型的糖苷键，具有三螺旋结构；扫描电镜观察WSP-3 呈大小不均一的棒状。抗氧化试验表明：WSP-3 对DPPH·、·OH 和ABTS+·具有一定的清除能力，IC50 分别为3.38、3.05、3.39 μg/mL。

中文关键词: 牡丹花蕊水溶性多糖纯化结构表征降血糖抗氧化

Abstract:The crude polysaccharides were extracted from stamens of Paeonia suffruticosa by ultrasonic complex enzyme and obtained by decolorization and proteinization. Three water -soluble polysaccharide（WSP）components（WSP-1，WSP-2 and WSP-3）were purified by DEAE-52 cellulose column chromatography，and were further purified by Sephadex G-100 gel chromatography with high yield and good hypoglycemic activity.The results showed that WSP-3 was an acidic homozygous polysaccharide with a molecular weight of 14 351 Da，and the residues contained α-glycosidic bonds with a triple helix structure. WSP-3 was observed by scanning electron microscope as a rod of uneven size. Antioxidant experiments showed that WSP-3 had certain scavenging ability on DPPH·，·OH，and ABTS+·，and the IC50 was 3.38，3.05 and 3.39 μg/mL，respectively.

keywords: water-soluble polysaccharides from stamens of Paeonia suffruticosa purification structural characterization hypoglycemic effect anti-oxidation

文章编号：202307012 中图分类号： 文献标志码：

基金项目:

作者	单位
段雪莹，罗磊*，李瀚姝，赵一帆，马潇	河南科技大学食品与生物工程学院，河南洛阳 471000

Author Name

Affiliation

DUAN Xue-ying，LUO Lei*，LI Han-shu，ZHAO Yi-fan，MA Xiao

The crude polysaccharides were extracted from stamens of Paeonia suffruticosa by ultrasonic complex enzyme and obtained by decolorization and proteinization. Three water -soluble polysaccharide（WSP）components（WSP-1，WSP-2 and WSP-3）were purified by DEAE-52 cellulose column chromatography，and were further purified by Sephadex G-100 gel chromatography with high yield and good hypoglycemic activity.The results showed that WSP-3 was an acidic homozygous polysaccharide with a molecular weight of 14 351 Da，and the residues contained α-glycosidic bonds with a triple helix structure. WSP-3 was observed by scanning electron microscope as a rod of uneven size. Antioxidant experiments showed that WSP-3 had certain scavenging ability on DPPH·，·OH，and ABTS+·，and the IC50 was 3.38，3.05 and 3.39 μg/mL，respectively.

引用文本：

用微信扫一扫