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投稿时间:2022-07-21
投稿时间:2022-07-21
中文摘要: 通过紫外-可见光谱法、荧光分光光度法分析不同pH 值(pH5、6、7)和不同复配摩尔比条件下绿原酸(chlorogenic acid,CA)与豌豆豆球蛋白(pea legumin protein,LG)的相互结合现象,并探究豌豆豆球蛋白对绿原酸光稳定性、热稳定性、酸碱稳定性的影响。结果表明,豌豆豆球蛋白可以与绿原酸发生非共价作用,不同pH 值条件下豌豆豆球蛋白-绿原酸复配物均发生荧光淬灭现象,荧光强度随绿原酸含量的增加而降低;豌豆豆球蛋白对绿原酸的热稳定性(50、80 ℃)和光稳定性(日光、紫外)有显著(P<0.05)提高作用;在常温(25 ℃)、避光以及pH6 条件下,由于绿原酸自身稳定而未见明显变化。
Abstract:The interaction of chlorogenic acid(CA)and pea legumin(LG)under different pH(pH5,6,7)conditions and different compound molar ratios was analyzed by UV-vis and fluorescence spectrophotometry.The effects of pea LG on photostability,thermal stability,and acid-base stability of CA were investigated.The results showed that there was a non-covalent effect between pea LG and CA.Fluorescence quenching occurred in pea LG-CA complex under different pH conditions,and the fluorescence intensity decreased with the increase of CA content.Pea LG improved the thermal stability(50,80 ℃)and photostability(sunlight,UV)of CA(P<0.05).Under normal temperature,dark and pH6 conditions,no significant change was observed due to the stability of CA.
keywords: chlorogenic acid(CA) pea legumin(LG) non-covalent interaction polyphenol stability active material carrier
文章编号:202307006 中图分类号: 文献标志码:
基金项目:2019 年度天津市“131”创新型人才团队项目(201926)
作者 | 单位 |
王慧婷1,薛良雨1,崔圆梦1,韩宇辰1,唐鑫1,崔佳熠1,王志伟1*,张民1,2,3* | 1.天津科技大学食品营养与安全国家重点实验室,天津 300457;2.天津农学院食品科学与生物工程学院,天津 300392;3.天津农学院农产品加工中外联合研究中心,天津 300392 |
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