大孔树脂纯化灵芝子实体和孢子粉中总三萜

祝朋玲1; 张静1; 李思雨1; 崔馨戈1; 袁抒捷1; 王雯1; 彭灿1; 2; 3; 4; 5*; 周俊6

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DOI:10.12161/j.issn.1005-6521.2023.04.012

食品研究与开发:2023,44(4):79-85

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大孔树脂纯化灵芝子实体和孢子粉中总三萜

祝朋玲1，张静1，李思雨1，崔馨戈1，袁抒捷1，王雯1，彭灿1，2，3，4，5*，周俊6^1,2,3,4,5,6

(1.安徽中医药大学药学院，安徽合肥 230012;2.药物制剂技术与应用安徽省重点实验室，安徽合肥 230012;3.中药复方安徽省重点实验室，安徽合肥 230012;4.安徽省中医药研究院中药资源保护与开发研究所，安徽合肥 230012;5.省部共建安徽道地中药材品质提升协同创新中心，安徽合肥 230012;6.6.安徽黄山云乐灵芝有限公司，安徽宣城 242600)

Purification of Total Triterpenoids from Fruiting Body and Spore Powder of Ganoderma lucidum by Macroporous Adsorption Resins

ZHU Peng-ling1，ZHANG Jing1，LI Si-yu1，CUI Xin-ge1，YUAN Shu-jie1，WANG Wen1，PENG Can1，2，3，4，5*，ZHOU Jun6^1,2,3,4,5,6

(1.School of Pharmacy，Anhui University of Chinese Medicine，Hefei 230012，Anhui，China;2.Anhui Province Key Laboratory of Pharmaceutical Preparation Technology and Application，Hefei 230012，Anhui，China;3.Anhui Province Key Laboratory of Chinese Medicinal Formula，Hefei 230012，Anhui，China;4.Institute of TCM Resources Protection and Development，Anhui Academy of Chinese Medicine，Hefei 230012，Anhui，China;5.MOE-Anhui Joint Collaborative Innovation Center for Quality Improvement of Anhui Genuine Chinese Medicinal Materials，Hefei 230012，Anhui，China;6.6.Anhui Huangshan Yunle Ganoderma Lucidum Co.，Ltd.，Xuancheng 242600，Anhui，China)

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投稿时间：2022-02-25

中文摘要: 研究比较大孔树脂对灵芝子实体和孢子粉两个不同部位中总三萜的吸附特性，优选其对灵芝总三萜的分离纯化工艺条件。采用紫外分光光度法测定灵芝子实体和孢子粉两个不同部位中总三萜的含量，选择8种大孔树脂，以总三萜吸附率和解吸率为指标，筛选最佳树脂型号，并考察最佳树脂纯化灵芝子实体和孢子粉中总三萜的工艺参数。结果表明：8种大孔树脂中，HPD-500型大孔树脂对灵芝子实体总三萜的吸附解吸效果最好，最佳纯化工艺条件为上样液浓度1.0 g干样/mL，最大上样量2 BV，用8.5 BV的75%乙醇进行洗脱，该工艺下灵芝子实体总三萜纯度达60.59%；而AB-8型大孔树脂对灵芝孢子粉总三萜的吸附解吸效果最好，且最佳纯化工艺条件为上样液浓度0.4 g干样/mL，最大上样量3 BV，用8.5 BV的95%乙醇进行洗脱，该工艺下灵芝孢子粉总三萜纯度达65.48%。综上，HPD-500型和AB-8型大孔树脂分别富集纯化灵芝子实体和灵芝孢子粉中总三萜的工艺效果显著，简单可行，为灵芝三萜的深入探究和工业化纯化灵芝子实体和孢子粉总三萜提供理论依据。

中文关键词: 灵芝子实体灵芝孢子粉总三萜大孔树脂分离纯化

Abstract:The purification process of total triterpenoids from the fruiting body and spore powder of Ganoderma lucidum by macroporous adsorption resins were optimized.Ultraviolet-visible（UV-Vis）spectrophotometry was employed to detect the content of total triterpenoids in the two different parts of G.lucidum.Eight different macroporous adsorption resins were used for the purification，and the optimum one was selected with the absorption rate and desorption rate of total triterpenoids as the indicators.The process of the purification with the optimum macroporous resin was optimized with the yield of total triterpenoids as the indicator.For the purification of total triterpenoids from G.lucidum fruiting body，the HPD-500 macroporous adsorption resin had the best adsorption and desorption performance among the eight resins.The optimal purification process was as follows:the sample concentration of1.0g/mL，the maximum loading amount of 2BV，and the elution with75% ethanol of8.5BV，under which the product showed the purity of 60.59%.For the purification of total triterpenoids from G.lucidum spore powder，the AB-8 macroporous adsorption resin showed the best adsorption and desorption performance among the eight resins.The optimal purification process was as follows:the sample concentration of 0.4 g/mL，the maximum loading amount of 3 BV，and the elution with 95% ethanol of 8.5 BV，under which the product showed the purity of 65.48%.In conclusion，HPD-500 and AB-8 macroporous adsorption resins can be used for the purification of total triterpenoids from G.lucidum fruiting body and spore powder，respectively.

keywords: Ganoderma lucidum fruiting body Ganoderma lucidum spore powder total triterpenoids macroporous adsorption resins separation and purification

文章编号：202304012 中图分类号： 文献标志码：

基金项目:安徽省科技重大专项项目（202103a07020001）；安徽省药品监督管理局科技创新项目（AHYJ-JK-202109）；安徽高校协同创新项目（GXXT-2019-043）

作者

单位

祝朋玲1，张静1，李思雨1，崔馨戈1，袁抒捷1，王雯1，彭灿1，2，3，4，5*，周俊6

1.安徽中医药大学药学院，安徽合肥 230012；2.药物制剂技术与应用安徽省重点实验室，安徽合肥 230012；3.中药复方安徽省重点实验室，安徽合肥 230012；4.安徽省中医药研究院中药资源保护与开发研究所，安徽合肥 230012；5.省部共建安徽道地中药材品质提升协同创新中心，安徽合肥 230012；6.安徽黄山云乐灵芝有限公司，安徽宣城 242600

Author Name

Affiliation

ZHU Peng-ling1，ZHANG Jing1，LI Si-yu1，CUI Xin-ge1，YUAN Shu-jie1，WANG Wen1，PENG Can1，2，3，4，5*，ZHOU Jun6

1.School of Pharmacy，Anhui University of Chinese Medicine，Hefei 230012，Anhui，China；2.Anhui Province Key Laboratory of Pharmaceutical Preparation Technology and Application，Hefei 230012，Anhui，China；3.Anhui Province Key Laboratory of Chinese Medicinal Formula，Hefei 230012，Anhui，China
4.Institute of TCM Resources Protection and Development，Anhui Academy of Chinese Medicine，Hefei 230012，Anhui，China；5.MOE-Anhui Joint Collaborative Innovation Center for Quality Improvement of Anhui Genuine Chinese Medicinal Materials，Hefei 230012，Anhui，China；6.Anhui Huangshan Yunle Ganoderma Lucidum Co.，Ltd.，Xuancheng 242600，Anhui，China

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