Abstract:The fungal diversity in the peel and core of Houhuo low-temperature Daqu was analyzed by Illumina MiSeq high-throughput sequencing for the internal transcribed spacer（ITS）sequence.The alpha diversity and richness of the fungi in the Daqu core were significantly higher（P<0.05）.The beta diversity analysis indicated that the fungal community structure was different between Daqu peel and Daqu core （P<0.001）.At the genus level，the dominant fungal genus in the Daqu peel was Saccharomycopsis （98.92%），while those in the Daqu core were Saccharomycopsis（58.20%）and Aspergillus（40.97%）.There were only 2 operational taxonomic units（OTUs）with average relative abundance>1.0%，which belonged to Saccharomycopsis（70.84%） and Aspergillus（17.91%），respectively.The linear discriminant analysis effect size（LEfSe）showed that Saccharomycopsis and Aspergillus were the key genera causing the difference between Daqu peel and Daqu core.The results demonstrated that the dominant fungal taxa in the Houhuo low-temperature Daqu were Saccharomycopsis and Aspergillus，and the fungal community structure varied between Daqu peel and Daqu core.The findings provide data support for the subsequent improvement of Daqu-making process and the mining of microbial resources for brewing in central China.