Abstract:The paper aimed at initially exploring the diversity of endophytic yeast in sugarcane and screening strains with good fermentation performance.The endophytic yeast in sugarcane was isolated by the tissue culture method，and then identified based on morphology，physiology，biochemistry，and 26S rDNA D1/D2 region analyses.Strains with good gas-producing performance were initially screened by the Duchenne tube fermentation method to determine their optimal growth conditions.Subsequently，the strains with excellent fermentation performance were further screened with sugar，salt，and alcohol tolerance tests by shaking-flask fermentation.Sixty-three strains of endophytic yeast were isolated and identified as eight species，namely Hanseniaspora uvarum，Meyerozyma guiliermondii，Debaryomyces hansenii，Candida oleophila，Candida zeylanoides，Wickerhamomyces anomalus，Cystobasidium minutum，and Papiliotrema terrestris.Among the above 63 strains，strain J-6 showed especially excellent growth and fermentation performance，whose growth rate reached the maximum（1.993）after 24 hours at 26 ℃ and pH5.0.It showed tolerance in the context of 20%ethanol，140 g/L sodium chloride，and 300 g/L glucose.Its OD600nmreached up to 1.935，1.608，and 1.901 under the conditions of 16%ethanol，60 g/L sodium chloride，and 150 g/L glucose，respectively，which was identified as W.anomalus.