Abstract:The parameters of enzymatic hydrolysis were explored for the production of Polygonatum sibiricum instant tea.With the yield of P.sibiricum instant tea as the response value and the liquid-to-material ratio，enzyme dosage，enzymatic hydrolysis temperature and time as the independent variables，the processing parameters of the instant tea were optimized by response surface methodology.The results showed that the optimum enzymatic hydrolysis parameters were liquid-to-material ratio of 21 ∶1（mL/g），enzyme dosage of 1.4%，and enzymatic hydrolysis at 47℃for 37 min.Under these conditions，the yield of P.sibiricum instant tea was 26.08%，which was close to the predicted value of 26.82%.The P.sibiricum instant tea extracted by ultrasound combined with compound enzyme was yellow，with the polysaccharide content of 3.73% and the flavonoid content of 0.95%.The IC50values of Polygonatum sibiricum instant tea for scavenging 1，1-diphenyl-2-picrylhydrazyl（DPPH）free radicals and superoxide free radicals were 14.34 mg/mL and 16.45 mg/mL，respectively.Moreover，the free radical scavenging ability of the instant tea increased with the increase in the concentration.