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投稿时间:2022-03-31
投稿时间:2022-03-31
中文摘要: 该文探究人参炮制品发酵前后功能性成分变化,以白参、红参及黑参为原料,利用复合菌(植物乳杆菌与肠膜明串珠菌肠膜亚种)对其进行发酵,测定理化指标及皂苷、氨基酸含量及功效酶活力。试验发现微生物对发酵底物的不断利用使各组发酵液中总糖含量逐渐降低,作为发酵代谢产物的总酚及总酸含量逐渐增加。在发酵前,白参中天然人参皂苷Rb2、Rb1、Re、Rc的含量较高。与白参相比,红参和黑参中主要天然人参皂苷含量减少,稀有皂苷有少量生成。发酵前,白参中游离氨基酸的总含量最高,而黑参中总氨基酸含量最低;发酵后,人参炮制品的游离氨基酸含量均出现大幅度减少。必需氨基酸总含量除白参增加外,红参和黑参的必需氨基酸总含量明显降低。检测发酵前后蛋白酶、脂肪酶和超氧化物歧化酶功效酶酶活力,3种功效酶酶活力均呈现不同程度的提升。结果表明,人参炮制品在发酵后功能性成分会发生较大变化,识别和确认其成分有助于人参的加工和流通。
Abstract:The changes of functional components of ginseng processed products before and after fermentation were studied.White ginseng,red ginseng and black ginseng were used as raw materials and fermented by compound bacteria (Lactobacillus plantarum and Clostridium enteroplast subspecies).The physical and chemical indexes,saponins,amino acids and activities of functional enzymes were determined.It was found that the continuous utilization of fermentation substrates by microorganisms resulted in a gradual decrease in the content of total sugar,and a gradual increase in the contents of total phenol and total acid as fermentation metabolites.Before fermentation,the contents of natural ginsenosides Rb2,Rb1,Re and Rc in white ginseng were relatively high.Compared with those in white ginseng,the contents of main natural ginsenosides in red ginseng and black ginseng were decreased,and a small amount of rare ginsenosides were produced.Before fermentation,the total content of free amino acids in white ginseng was the highest,while that in black ginseng was the lowest.After fermentation,the content of free amino acids in ginseng processed products dropped.The total content of essential amino acids in red ginseng and black ginseng was decreased obviously while that in white ginseng was increased.Determination of the activities of protease,lipase and superoxide dismutase before and after fermentation demonstrated that the activities of the three enzymes were enhanced in different degrees.The results showed that the functional components of ginseng processed products changed greatly after fermentation,and thus identifying and confirming the components was helpful for the processing and circulation of ginseng.
文章编号:202301007 中图分类号: 文献标志码:
基金项目:吉林省科技发展计划项目(20210204179YY)
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