Abstract:Ultrasound-assisted Tween 80 was used to optimize the extraction of gamma-amino butyric acid（GABA）and polyphenols from germinated millet.Taking GABA and polyphenol yield as the inspection index，the influence of Tween 80 mass fraction，ultrasonic temperature and time，solid-liquid ratio，and ultrasonic power on the GABA yield were investigated using the single factor test，and an orthogonal test was designed to optimize the extraction process of GABA.The effects of Tween 80 mass fraction，ultrasonic temperature and time，and solid-liquid ratio on the yield of polyphenols were also studied using the single factor test，and the extraction process of polyphenols was optimized through response surface design.The results showed that the optimum technological parameters of ultrasound-assisted Tween 80 to extract of GABA were:a Tween 80 mass fraction of 6%，ultrasonic temperature of 35 ℃，ultrasonic time of 25 min，solid-liquid ratio of 1∶18（g/mL），and ultrasonic power of 126 W.Under these conditions，the final GABA yield was 4.70 mg/g.The optimum technological parameters for extracting polyphenols were:a Tween 80 mass fraction of 9%，ultrasonic temperature of 42 ℃，ultrasonic time of 19 min，and solid-liquid ratio of 1∶12.5（g/mL）.Under these conditions，the final polyphenol yield was 1.99 mg/g.