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投稿时间：2022-02-20
投稿时间：2022-02-20
中文摘要: 莲子抗性淀粉（lotus seed resistant starch，LRS）是一种可以促进双歧杆菌增殖的良好碳源，其可在被利用前与菌发生物理性黏附接触，这种黏附接触可能会促进双歧杆菌增殖进而使二者黏附体系在人体中发挥益生功效，然而目前对二者黏附能力的影响因素及黏附机理尚不清楚。因此该研究以微波法制备Ⅲ型莲子抗性淀粉（LRS3），并通过不同的酶、胆盐浓度、pH值条件、生长介质、温度的处理以及测定二者的疏水性和表面电荷，研究LRS3与双歧杆菌黏附的影响因素。结果表明LRS3与双歧杆菌黏附体系在通过胃肠道过程中损失较大，静电相互作用对其存在一定的影响，疏水相互作用对二者黏附体系的影响存在菌株差异性。推测该黏附作用机理可能涉及一类特异性的细胞表面蛋白。
Abstract:Resistant starch from lotus seeds is a good carbon source that promotes the proliferation of Bifidobacteria.The starch makes physical adhesive contact with the bacteria before mediating its effects.This adhesion contact may promote the proliferation of Bifidobacteria and，thus，this adhesion system may have a probiotic effect in humans.However，the factors affecting the adhesion ability and adhesion mechanism of the starch and bacteria remain unclear.Therefore，in this study，lotus seed resistant starch（LRS3）was prepared using the microwave method，and the factors affecting the adhesion of LRS3 to Bifidobacteria were studied using different enzymes，bile salt concentrations，pH conditions，growth media，and temperatures.Moreover，their hydrophobicity and surface charges were determined.The results showed that the adhesion system of LRS3 and Bifidobacteria was adversely affected by passage through the gastrointestinal tract.Electrostatic interaction had a certain influence on adhesion，and the influence of hydrophobic interactions on adhesion differed between different bacterial strains.The study speculated that the adhesion mechanism may involve a specific class of cell surface proteins.
keywords: lotus seed resistant starch Bifidobacterium adhesion effect adhesion effect influencing factors prebiotic effect
文章编号：202224016 中图分类号： 文献标志码：
基金项目:福建农林大学国际科技合作与交流项目（KXGH17001）；福建省自然科学基金项目（2019J01391）；福建省特种淀粉品质科学与加工技术重点实验室开放课题（FJDF201807）
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