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投稿时间：2021-08-26
投稿时间：2021-08-26
中文摘要: 为探究微波-压热法制备马蹄抗性淀粉的最优工艺条件，该文以马蹄淀粉为原料，分别考察淀粉乳浓度、老化时间、微波时间、老化温度4个单因素对马蹄抗性淀粉得率的影响。选取淀粉乳浓度、老化时间、微波时间进行响应面工艺优化，并测定抗性淀粉与原淀粉的理化性质和结构特征。结果表明：马蹄抗性淀粉的最佳制备工艺为淀粉乳浓度23%、微波时间74 s、121℃下压热40 min，4℃下老化12 h。在该条件下，抗性淀粉得率为16.85%，模型预测值为16.89%，其相对误差＜0.5%，验证响应面模型与实际情况得到了良好拟合，说明通过响应面方法得到的优化工艺非常可靠。理化性质测定发现马蹄抗性淀粉的溶解度、膨润度、冻融性显著高于原淀粉，而其持水性却低于原淀粉。
Abstract:To explore the optimal conditions for preparing resistant starch from waterchestnut with microwaveautoclave method，this study used the starch from waterchestnut as the raw material and investigated the effects of starch milk concentration，aging time，microwave time and aging temperature on yield of resistant starch.The four factors above were optimized via response surface methodology，and the physicochemical properties and structural characteristics of the resistant starch and the original starch were determined.The results showed that the preparation conditions were optimized as follows：starch milk concentration of 23%，microwave time of 74 s，autoclave at 121℃ for 40 min and retrogradation at 4℃ for 12 h.Under these conditions，the maximum yield of resistant starch was 16.85%，which showed the relative error＜0.5%in comparison with the model predicted value（16.89%）.The results verified that the preparation conditions optimized by response surface methodology well fitted to the actual situation and were reliable.The resistant starch from water chestnuthad significantly higher solubility，swelling capacity，and freeze-thaw property and lower water-holding capacity than the original starch.
keywords: resistant starch from water chestnut response surface microwave-autoclave method yield physicochemical properties
文章编号：202221010 中图分类号： 文献标志码：
基金项目:国家自然科学基金项目（31860457）；广西自然科学基金青年基金项目（2018GXNSFBA281118、2019GXNSFBA245037、2020GXNSFAA297153、2020GXNSFBA297111、2020GXNSFAA297007）；广西高等教育本科教学改革工程项目（2021JGZ160）；广西高校中青年教师科研基础能力提升项目(2021KY0705)；贺州学院博士科研启动项目（HZUBS202008）
| 作者 | 单位 |
| 刘云芬1，2，郭珍红1，殷菲胧1，廖玲燕1，宋慕波1，2，帅良1，2* | 1.贺州学院食品与生物工程学院/食品科学与工程技术研究院，广西 贺州 542899；2.贺州学院广西康养食品科学与技术重点实验室，广西 贺州 542899 |
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