Abstract:Taking Inonotus obliquus as the research object，this research adopted the way of plate culture and liquid shake flask culture to observe and compare the effects of different carbon sources （maltose，glucose，sucrose，corn starch，lactose，corn flour，fructose，mannitol，sweet potato starch and soluble starch）on the biological characteristics of mycelium and the activity of liquid fermentation products.The results of solid culture showed that fructose，maltose and sucrose had a great influence on the growth rate of mycelium.When maltose was the carbon source，the mycelium grew fastest，reaching 0.302 cm/d.Fructose and sucrose delayed the aging of mycelium，the mycelia of corn flour and glucose were the strongest，and the mycelia of maltose and sweet potato starch had the highest growth density（the dense part was felt like wool shaped）.At the same time，the results of liquid fermentation showed that the total biomass of corn flour was the largest，followed by glucose.As for the active substances，most carbon sources promoted the accumulation of active substances with the extension of culture time.With glucose as the carbon source，the polysaccharide content of mycelium was the highest at 11 d，followed by lactose.When sweet potato starch，corn starch，maltose，and lactose were used as carbon sources，the 1，1-diphenyl-2-picrylhydrazyl（DPPH）free radical scavenging rate of water extract of mycelium reached more than 70%.