本文已被：浏览 1094次 下载 712次
投稿时间：2022-02-14
投稿时间：2022-02-14
中文摘要: γ-聚谷氨酸（γ-polyglutamic acid，γ-PGA）是一种新型绿色高分子材料，被广泛应用于农业生产、食品、医药等众多领域。目前γ-PGA生产成本高，产量低等问题较为突出。为降低生产成本，该文以廉价甘蔗糖蜜作为碳源。利用单因素与响应面法优化发酵培养基。结果显示，最佳培养基组成为糖蜜可溶性固形物浓度8.68%、酵母膏浓度4.23 g/L、FeSO4·7H2O 浓度 0.78 g/L，味精浓度 80 g/L，γ-PGA 产量为（67.88±0.41）g/L，与预测值 67.17 g/L 非常接近，相较于优化前γ-PGA产量提高了1.19倍，为工业化生产奠定基础。
Abstract:γ-Polyglutamic acid （γ-PGA）is a novel eco-friendly polymer.It has been widely used in agriculture，food，medicine，and many other industries.To date，the major problem with γ-PGA is its high production cost with low yield.To reduce the production cost，cane molasses （a low cost product）was selected as the carbon source for fermentation，and single factor test and response surface methodology were used to optimize the fermentation medium.The results showed that the optimal medium composition was molasses with 8.68% soluble solid，4.23 g/L yeast extract，0.78 g/L FeSO4·7H2O and 80 g/L monosodium glutamate.Under the above conditions，the yield of γ-PGA reached（67.88±0.41）g/L，which was similar to the predicted value of 67.17 g/L.The yield increased by about 1.19 times compared with the control.This result could lay the foundation for industrial production.
keywords: medium composition γ-polyglutamic acid（γ-PGA） response surface optimization cane molasses Bacillus licheniformis
文章编号：202220024 中图分类号： 文献标志码：
基金项目:宁夏回族自治区重点研发计划（2021BEG03011）；四川省科技计划重点研发项目（2021YFN0135）
引用文本：
津ICP备20000603号-1