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投稿时间:2021-08-06
投稿时间:2021-08-06
中文摘要: 以采集的25个野生灵芝子实体及其驯化栽培后的子实体为研究对象,分别采用苯酚-硫酸法、香草醛-高氯酸-冰乙酸显色法、凯氏定氮法、考马斯亮蓝法、马弗炉灼烧法、DPPH自由基清除试验和总还原力测定试验等测定灵芝多糖、三萜、粗蛋白、可溶性蛋白、灰分和体外抗氧化活性,并比较分析其含量差异。结果表明,不同灵芝菌株之间及同一菌株驯化前后子实体之间营养成分含量存在显著性差异,大部分野生灵芝的多糖和灰分含量高于其驯化后子实体,如野生灵芝多糖含量最高达57.30%,驯化后最高仅为49.40%;而大部分菌株驯化后子实体的三萜、粗蛋白和可溶性蛋白含量高于野生灵芝,如野生灵芝可溶性蛋白含量最高为6.29 mg/g,驯化后最高可达10.04 mg/g;驯化前水提液的体外抗氧化活性均高于驯化后子实体。
Abstract:The polysaccharide,triterpenoids,crude protein,soluble protein,ash,and antioxidant activity of 25 wild and domesticated Ganoderma lucidum fruiting bodies were determined using phenol-sulfuric acid extraction,vanillin-perchloric acid-glacial acetic acid colorimetry,Kjeldahl nitrogen determination,Coomassie brilliant blue staining,a muffle furnace,and the 1,1-diphenyl-2-picrylhydrazyl free radical scavenging test coupled to a total reducing power test,respectively.Significant differences in nutritional components were observed between different G.lucidum strains and between the fruiting bodies of the same strain before and after domestication.The polysaccharide and ash contents of most wild G.lucidum strains were higher than those of fruiting bodies after domestication.For example,the maximum polysaccharide content of wild Ganoderma strains was approximately 57.30%,whereas the value was only 49.40% after domestication.The contents of triterpenoids,crude protein,and soluble protein in the fruiting bodies of most strains after domestication were higher than those of wild G.lucidum.For example,the maximum soluble protein content of wild G.lucidum was 6.29 mg/g but only 10.04 mg/g after domestication.The antioxidant activity of water extracts before acclimation was higher than that of after acclimation.
keywords: wild Ganoderma lucidum domestication cultivation polysaccharide triterpenoids crude protein soluble protein ash antioxidant activity
文章编号:202220021 中图分类号: 文献标志码:
基金项目:河北省现代农业产业技术体系食用菌创新团队建设专项资金(HBCT2018050202);邯郸市科学技术研究与发展计划项目(21422012325)
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