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食品研究与开发:2022,43(17):127-133
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薏苡仁乳酸菌发酵工艺优化及活性成分变化分析
(1.贵州医科大学环境污染与疾病监控教育部重点实验室,贵州 贵阳 550025;2.贵州医科大学公共卫生与健康学院,贵州 贵阳 550025;3.贵州中烟工业有限公司技术中心,贵州 贵阳 550000)
Optimization of Lactic Acid Bacteria Fermentation of Coix Seed and Analysis of Its Active Components
(1.Key Laboratory of Environmental Pollution Monitoring and Disease Control,Ministry of Education,Guizhou Medical University,Guiyang 550025,Guizhou,China;2.School of Public Health,Guizhou Medical University,Guiyang 550025,Guizhou,China;3.Technology Center of China Tobacco Guizhou Industrial Co.,Ltd.,Guiyang 550000,Guizhou,China)
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投稿时间:2021-07-19    
中文摘要: 为得到薏苡仁发酵工艺的最优参数,该试验先通过测定薏苡仁发酵浆液中的pH值、总酸、还原糖、菌落总数等指标,确定最优发酵菌种;然后采用单因素和正交试验方法优化发酵工艺,分析薏苡仁发酵浆液中的总酚、β-葡聚糖在发酵过程中的变化规律。结果表明:适合于薏苡仁发酵的最优发酵菌种为植物乳杆菌;最佳发酵工艺条件为薏苡仁粉与水料液比1∶7(g/mL)、α-淀粉酶质量分数0.1%、接种量1%、发酵温度为37℃。薏苡仁发酵浆液中总酚和β-葡聚糖含量在发酵25 h时达到最高。
中文关键词: 薏苡仁  乳酸菌  发酵工艺  优化  活性成分
Abstract:To obtain the optimum parameters of Coix seed fermentation technology,the pH,total acid,reducing sugar and the total number of colonies in the fermentation broth of Coix seed were explored in this experiment to determine the optimal fermentation strain.Single factor test and orthogonal test were used to optimize the fermentation technology,and the changes of total phenols and β-glucan in the fermentation broth of Coix seed during fermentation were analyzed.The results showed that the strain suitable for Coix seed fermentation was Lactobacillus,and the optimal fermentation conditions were Coix seed powder-to-water of 1 ∶7(g/mL),αamylase mass fraction of 0.1%,and inoculation amount of 1%.Total phenols and β-glucan in the fermentation broth of Coix seed had the highest content 25 h after fermentation,and the curve was inverted U-shaped in the temperature gradient.
文章编号:202217018     中图分类号:    文献标志码:
基金项目:贵州医科大学乡村经济振兴研究与农业产业技术攻关项目(KCCG2021-11);贵州省科技计划项目(黔科合基础[2019]1254号);贵州省高等学校工程研究中心(黔教合KY字[2020]014)
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