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投稿时间:2021-07-15
投稿时间:2021-07-15
中文摘要: 为研究六堡茶中真菌的多样性及真菌毒素残留量情况,验证其饮用安全性,该试验选取10批次不同年份、不同企业生产的梧州六堡茶样品进行研究,先提取样品的总DNA,再利用高通量测序技术进行ITS测序分析,然后采用QuEChERS(quick、easy、cheap、effective、rugged、safe)-超高效液相质谱联用方法测定样品中 16 种常见真菌毒素等残留量。研究结果表明,梧州六堡茶中真菌的优势菌为曲霉属(Aspergillus)、青霉属(Penicillium),10个样品全部含有曲霉属,其平均相对含量为50.6%~99.9%,10个样品曲霉属平均相对含量86.4%;试验样品中真菌毒素残留量全部低于方法检出限。
中文关键词: 梧州六堡茶 高通量测序 真菌多样性 高效液相色谱-质谱联用 真菌毒素残留
Abstract:This study aimed to explore the diversity of fungi and mycotoxin residues in Liupao tea and test the safety of the tea.Specifically,ten batches of Wuzhou Liupao tea samples produced by different manufactures at different time were studied.The total DNA of the samples was extracted followed by high-throughput sequencing of ITS.Then quick,easy,cheap,effective,rugged,safe(QuEChERS)-ultra high performance liquid chromatographymass spectrometry was employed for the determination of sixteen common mycotoxinse.The results showed Aspergillus and Penicillium dominated the fungi in the tea and Aspergillus was found in all the ten batches of samples,with relative content of 50.6%-99.9%(mean:86.4%).Mycotoxin residues in all test samples were below the detection limit of the method.
keywords: Wuzhou Liupao tea high-throughput sequencing fungal diversity high performance liquid chromatography-mass spectrometry(HPLC-MS) mycotoxin residues
文章编号:202215027 中图分类号: 文献标志码:
基金项目:梧州学院重点科研项目(2020B008);广西高校中青年教师科研基础能力提升项目(2021KY0674);梧州市茶产业发展服务中心、梧州学院产学研项目(六堡茶产业发展评价与提质增效研究);2021年度广西中央引导地方科技发展资金项目(桂科ZY21195056)
作者 | 单位 |
梁剑锋1,2,李亚1,2*,宾月景1,2,奚广生1,2,陈金辉1,2,曾勇谋1,2,陈美华1,2 | 1.梧州学院食品与制药工程学院(六堡茶现代产业学院),广西 梧州 543002;2.广西六堡茶产业科研人才小高地,广西 梧州 543002 |
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