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食品研究与开发:2022,43(14):199-203
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酸菜中乳酸菌生长曲线、产酸性能及抑菌性能探究
(1.四川轻化工大学生物工程学院,四川 宜宾 644000;2.四川省远达集团富顺县美乐食品有限公司,四川 自贡 643200)
Investigation into the Growth Curve,Acid-production Ability,and Bacterial Inhibition of Lactic Acid Bacteria in Sauerkraut
(1.College of Bioengineering,Sichuan University of Science and Engineering,Yibin 644000,Sichuan,China;2.Sichuan Yuanda Group Fushun County Meile Food Co.,Ltd.,Zigong 643200,Sichuan,China)
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投稿时间:2021-10-18    
中文摘要: 针对自然发酵酸菜发酵时间长、菜叶易腐烂等问题,从自然发酵酸菜中筛选出产酸能力强及抑菌效果好的菌株人工接种到芥菜中,从而缩短发酵时间,改善酸菜的品质。以从自然发酵酸菜中筛选出的食品乳杆菌、植物乳杆菌和类干酪乳杆菌为研究对象,通过测定目标菌株在发酵液中发酵32 h过程中pH值的变化来反映目标菌株的产酸性能,采用牛津杯琼脂扩散法测定目标菌株对大肠杆菌、沙门氏菌和金黄色葡萄球菌3种指示菌的抑菌圈大小来反映目标菌株的抑菌性能,综合结果筛选出植物乳杆菌和类干酪乳杆菌作为人工添加菌株。
中文关键词: 酸菜  乳酸菌  生长曲线  产酸性能  抑菌特性
Abstract:In view of the problems of long fermentation time and perishable leaves of naturally fermented sauerkraut,strains with strong acid-production ability and good bacterial inhibition were extracted from naturally fermented sauerkraut and artificially inoculated into mustard to shorten the fermentation time and improve the quality of sauerkraut.The fluctuation of pH levels during 32-hour fermentation of Lactobacillus alimentarius,L.plantarum,and L.casei screened out from naturally fermented sauerkraut in the fermentation broth was measured,which reflected the acid-production performance of the strains.The inhibition circle sizes of the target strains against three indicator bacteria,namely Escherichia coli,Salmonella,and Staphylococcus aureus,were measured by the Oxford penicillin cup method,which reflected the inhibition performance of the target strains.The results showed that L.plantarum and L.casei were screened out as artificially added strains.
文章编号:202214025     中图分类号:    文献标志码:
基金项目:自贡市重点科技计划项目(2018CGZH10)
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