Abstract:Functional proteins are highly effective in disease prevention and health improvement.However，their low bioavailability has hindered their scope of use.The chitooligosaccharides-Tremella fuciformis polysaccharide（COS-TFP）of nanocomposites of loaded bovine serum albumin（BSA）protein was constructed by static interaction.The stability of the nanocomposites was investigated using particle size，the polydispersity index，and potential as indicators.BSA was used as a model protein to study the loading and release characteristics of the composite system.The total concentration of COS and TFP was 2 mg/mL.The concentration ratio of COS/TFP was 1∶5-1∶3.The concentration of NaCl ranged from 20 mmol/L to 50 mmol/L at pH 4.0.Under these conditions，particle size，multi-dispersion index，and potential were all within the stable range，and the COS-TFP of the nanocomposites was extremely stable.When the COS-TFP∶BSA ratio was 1∶0.6，the burial rate was 91%.BSA release was low at pH 4.0（full stomach environment）and rapid at 7.0（intestinal environment）.These findings indicated that the chitooligosaccharide polyelectrolyte nanocomposite system can be used as a potential proteincontrolled release delivery system.