Abstract:The regulation of gastrointestinal motility by spore-forming microbes and the potential mechanism were investigated.Spore-forming microbes were extracted in vitro from the feces of healthy volunteers using the chloroform.The extraction efficiency was confirmed by polymerase chain reaction（PCR）.The pseudo germ-free mouse model created using the antibiotic cocktail approach was evaluated by a fecal bacteria suspension coating experiment and Enterobacterial Repetitive Intergenic Consensus Sequences-PCR（ERIC-PCR）.The fecal suspension coating plate collected and processed on day 11 of PGF mice modeling did not produce visible colonies.The ERIC-PCR bands were greatly diminished，demonstrating the successful establishment of the model.The mice were randomly divided into model and spore-forming microbe groups.Transplantation in the spore-forming microbes group was performed by gavage.The colonization of spore-forming microbes was confirmed by quantitative PCR.Intestinal thrust and residual gastric rates were used to assess intestinal motility.The contents of 5-hydroxytryptamine（5-HT，serotonin）and tryptophan hydroxylase 1（TPH1）in the colon and plasma were determined.Following colonization，the residual gastric rate of mice was significantly decreased（P＜0.05），while the intestinal thrust rate was significantly increased （P＜0.01）.The contents of Ruminococcus，Ruminococcus_torques_group，and Dorea in feces were significantly increased.The levels of 5-HT in the colon and plasma were significantly increased，but the expression of TPH1 in the colon was significantly downregulated.The results indicate that spore-forming microbes promote gastrointestinal motility by increasing intestinal and plasma serotonin levels.