Abstract:This study aimed to improve the stability and bioavailability of isothiocyanate in Moringa oleifera seeds.To be specific，the flexible liposome was prepared with the thin film dispersion method.With the evaluation index of entrapment efficiency，the mass ratio of lecithin to cholesterol，mass ratio of lecithin to sodium cholate，and ultrasonic time in the preparation of the flexible liposome of M.oleifera seed isothiocyanate were optimized by single factor test and response surface methodology.The free isothiocyanate in liposomes was separated by low-temperature ultracentrifugation，and isothiocyanate content was determined by UV spectrophotometry.The results showed that the optimized process was as follows：lecithin-to-cholesterol mass ratio of 9.95∶1，lecithin-to-sodium cholate mass ratio of 10.32∶1，and ultrasonic time of 7.78 min.Under these conditions，the entrapment efficiency was calculated to be 64.17%.Moreover，the yielded isothiocyanate-loaded liposomes had the particle size of 348 nm，polydispersity index（PDI）of 0.251，and the Zeta potential of-24.7 mV.Transmission electron microscopy（TEM）showed that the liposomes were fairly uniform and regular round.After 24 h，the in vitro release rate of the liposomes was 80.105%，marking the ideal slow-release effect of the liposomes.