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投稿时间:2021-06-12
投稿时间:2021-06-12
中文摘要: 该研究以采集的4个黄酒麦曲样品为研究对象,利用Illunima Miseq高通量测序技术对麦曲样品中真菌的群落结构和多样性进行解析。研究发现,Ascomycota(子囊菌门)是麦曲中的主要菌门,平均相对含量高达90.21%;曲霉属(Aspergillus)、酵母属(Saccharomyces)、横梗霉属(Lichtheimia)、根霉属(Rhizopus)、犁头霉属(Absidia)和脉孢菌属(Neurospora)为麦曲中的优势细菌属,其中曲霉菌属(Aspergillus)的平均相对含量高达68.21%。在操作分类单元(operational taxonomic unit,OTU)水平上,OTU3213、OTU3344、OTU4507、OTU623 和 OTU3471 为麦曲中的优势 OTU,其中属于Aspergillus的OTU3213平均相对含量高达64.14%。由此可知,黄酒麦曲中含有大量的核心真菌微生物。
中文关键词: 麦曲 Illunima Miseq高通量测序 真菌 群落结构 多样性
Abstract:In this study,four wheat starter samples used for yellow wine production were collected and the fungal community structure and diversity were analyzed by high-throughput sequencing using the Illunima Miseq platform.The results showed that Ascomycota was the dominant fungal phylum in the wheat starters,with an average relative abundance of up to 90.21%.Moreover,Aspergillus,Saccharomyces,Lichtheimia,Rhizopus,Absidia,and Neurospora were the dominant fungal genera in the wheat starters.The average relative abundance of Aspergillus was as high as 68.21%.At the operational taxonomic unit(OTU)level,OTU3213,OTU3344,OTU4507,OTU623,and OTU3471 were the dominant OTUs.OTU3213,which is affiliated with Aspergillus,had an average relative abundance of up to 64.14%.Thus,it can be concluded that the wheat starters used for yellow wine production contained a large number of core fungal microorganisms.
keywords: wheat starter Illunima Miseq high throughput sequencing fungal community structure diversity
文章编号:202212024 中图分类号: 文献标志码:
基金项目:襄阳市成果转化领域重点科技计划项目(2020AAT00469);百校联百县-高校服务乡村振兴科技支撑行动计划
作者 | 单位 |
席啦1,2,孔祥聪1,2,杨少勇3,刘忠军2,郭壮1,2,赵慧君1,2* | 1.湖北文理学院湖北省食品配料工程技术研究中心,湖北 襄阳 441053;2.襄阳市酿酒生物技术与应用企校联合创新中心,湖北 襄阳 441053;3.湖北古襄阳酒业有限公司,湖北 襄阳 441100 |
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