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投稿时间：2021-12-08
投稿时间：2021-12-08
中文摘要: 以D101大孔树脂对苦丁茶粗提物中的总黄酮进行分离纯化，确定最佳纯化工艺条件，测定纯化前后的样品总黄酮的纯度，并检测纯化前后样品对黄嘌呤氧化酶（xanthine oxidase，XOD）的体外抑制活性。通过单因素试验得到最佳优化工艺条件为上样液pH值为3、洗脱液乙醇浓度70%、上样液浓度3.0 mg/mL、上样液流速2.0 mL/min、洗脱液流速2.0 mL/min、上样量55 mL、洗脱液体积40 mL。纯化后样品纯度由75.53%提高至94.40%，对XOD的IC50分别为（228.22±1.07）μg/mL 和（135.74±1.02）μg/mL。
Abstract:In this study，the total flavonoids in Kudingcha crude extract were separated and purified using D101 macroporous resin.The optimum purification conditions were elucidated，the purity of the total flavonoids before and after purification was determined，and the in vitro inhibitory activity of the samples on xanthine oxidase（XOD）was detected.The optimum process conditions were determined using Harman's single factor test，and the results were as follows：sample pH value 3；eluent 70%ethanol solution；concentration of sample solution 3.0 mg/mL；flow rate of sample solution 2.0 mL/min；flow rate of eluent 2.0 mL/min；sample volume 55 mL；and eluent volume 40 mL.Following purification，the purity of the sample was increased from 75.53%to 94.40%.The IC50values for XOD inhibition were（228.22±1.07）μg/mL before purification and（135.74±1.02）μg/mL after purification，respectively.
keywords: flavonoids of kudincha macroporous resin purification process xanthine oxidase inhibitory activity
文章编号：202209017 中图分类号： 文献标志码：
基金项目:广西农业科技创新联盟“农产品精深加工产业科技先锋队”
| Author Name | Affiliation |
| TAN Song-yan，YANG Zhi-wei | College of Light Industry and Food Engineering，Guangxi University，Nanning 530004，Guangxi，China |
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