Abstract:Broccoli sprouts are a very nutritious source of many beneficial ingredients and have been widely studied in recent years.The anti-tumor activity and potential mechanism of broccoli sprout extract were investigated in this study.Medium and high concentrations of broccoli sprout extract could reduce the cell density of Caco-2 and HepG2 cells.Signs of apoptosis were evident in images.Broccoli sprout extract inhibited the proliferation of the proliferation of Caco-2 and HepG2 tumor cell lines in a dose-dependent manner，with a 50%inhibition concentration in the presence of CaCl2（0.030±0.002）mg/mL and （0.043±0.004）mg/mL，respectively.The apoptosis rate detected by flow cytometry reached 82.95%in samples treated with the extract.Measurements of reactive oxygen species（ROS）and mitochondrial membrane potential showed that Caco-2 cells were sensitive to broccoli sprout extract，which could induce Caco-2 cell apoptosis by inducing the production of reactive oxygen species and reducing mitochondrial membrane potential.