Abstract:Seeds from six kinds of cruciferous vegetables—turnip，leaf mustard，tuber mustard，cabbage，cauliflower，and broccoli—were used to study the effects of hydrolysis followed by extraction（HFE）and simultaneous hydrolysis and extraction（SHE）on isothiocyanate（ITC）types and their contents measured through cyclization and gas chromatography-mass spectrometry（GC-MS）.The effects of extracts obtained using different extraction methods on B16 cell activity and NAD（P）H：quinone oxidoreductase 1（NQO1）activity in seeds were also analyzed.SHE resulted in significantly higher ITC contents measured in broccoli and turnip seeds than HFE（P＜0.05）.GC-MS measurements showed that SHE resulted in significantly higher contents of sulforaphane（3.26-fold），erucin，raphasatin，4-MI，and B，4-I-1 in turnip seeds；of erucin（1.63-fold）in broccoli seeds；and of iberverin in leaf mustard，cabbage，cauliflower，and broccoli seeds than HFE（P＜0.05）.SHE also resulted in higher contents of iberverin and octane measured in mustard and broccoli seeds，respectively，and lower contents of cyclopropyl isothiocyanate measured in cauliflower seeds than HFE.However，SHE had no significant effect on the contents of other ITC types such as alyssin and iberin（P＞0.05）.Furthermore，the rates of inhibition of B16 cell activity by turnip，cabbage，cauliflower，and broccoli seed extracts in the SHE group were significantly higher than those in the HFE group（P＜0.05）.The NQO1 activity of broccoli seeds in the SHE group was also significantly higher than that in the HFE group（P＜0.05）.