Abstract:Bacillus subtilis was used for fermentation of shrimp heads，and the effects of B.subtilis solid-state fermented shrimp head inoculant on the growth indexes and intestinal microflora of mice were investigated.Mice were randomly assigned into maintenance diet control group（NC），maintenance diet+0.1% unfermented shrimp head group（MC），and maintenance diet+0.1% fermented shrimp head inoculant group（MH）.The feeding experiment lasted for 8 weeks.The results showed that both unfermented and fermented shrimp heads had food calling effect.The body fat rate of MC group was higher than that of NC group，while MH group had a body fat rate 62.9% lower than NC group（P<0.05）.The spleen index of MH group increased by 35.7% compared with that of NC group（P<0.05）.Compared with that of NC group，the relative abundance of beneficial microorganisms such as Lactobacillus reuteri and L.johnsonii in the intestinal flora of MC and MH groups increased，and the probiotic effect in MH group was better.The results indicated that shrimp heads induced the food intake.Direct feeding of unfermented shrimp heads would increase the body fat rate of mice，while the shrimp heads fermented with B.subtilis could significantly reduce the body fat rate.The addition of 0.1% fermented shrimp head inoculant in the diet could increase the relative abundance of beneficial microorganisms such as lactic acid bacteria in the intestine and improve the immunity of mice.