Abstract:This study aimed to test the effects of synbiotics on the antioxidant capacity and intestinal flora of mice.Forty-eight male BALB/c mice were randomly assigned into the control （normal saline）group and lowdose（L），medium-dose（M），and high-dose（H）synbiotic groups and administrated with corresponding agents by gavage.After 14 days，the feces were collected for the characterization of intestinal flora changes by colony counting and 16S rDNA high-throughput sequencing.The antioxidant indexes in the liver and small intestine tissues and the levels of interleukin-6（IL-6）and interleukin-1β（IL-1β）in the serum were measured.In the small intestine and liver tissues，the activities of total superoxide dismutase（T-SOD），glutathione peroxidase（GSH-Px），and glutathione（glutathione，GSH）rose，and the malondialdehyde（MDA）content reduced after the administration of synbiotics.The level of IL-6 in group H declined（p<0.01）.The number of lactobacilli and bifidobacteria in each synbiotics group increased after 14 days of administration.The 16S rDNA high-throughput sequencing results demonstrated that the administration of synbiotics increased the abundance of Rikenella，Alistipes，Bifidobacterium and Lactobacillus.The administration of the above-mentioned synbiotics could improve the antioxidant capacity，reduce the levels of inflammatory cytokines，increase the number of lactobacillus and bifidobacteriain the intestine，and regulate the intestinal flora.