Abstract:The conditions for alleviating brown rice retrogradation using crude enzyme mixture derived from the fermented broth of Ganoderma lucidum were optimized.Firstly，the activities of α-amylase，β-amylase，cellulase，and acid protease were determined in the crude enzyme mixture.The variables including the reaction temperature，reaction duration，and liquid-solid ratio were modulated individually，and retrogradation value ratio of brown rice for each condition was determined，thereby elucidating the optimal conditions for alleviating brown rice retrogradation.The results showed that the activities of various enzymes in the crude enzyme mixture were significantly lower than those of intracellular enzymes present in G.lucidum.Moreover，these enzyme showed their peak activity on the 4th day with α-amylase，cellulase，and acid protease showing activities of（69 496.21±1 250.72），（75 517.73±2 142.82）and（7 515.79±204.08）U/g，respectively.Thus，the optimal condition for alleviating brown rice retrogradation were determined as:reaction temperature 32 ℃，reaction duration 2 h，and liquid-solid ratio 4∶1（mL/g）；the rate of regeneration value was the highest at 24.0%.Collectively，the results show that the crude enzyme mixture derived from fermented G.lucidum can alleviate brown rice retrogradation and provide a theoretical basis for expanding the application of brown rice.