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食品研究与开发:2022,43(6):68-74
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响应面法优化花椒叶多酚提取工艺及其抗氧化活性
(1.苏州农业职业技术学院 食品科技学院,江苏 苏州 215008;2.河南科技学院 食品学院,河南 新乡 453003)
Optimization of Extraction Process of Polyphenols from Zanthoxylum bungeanum Leaf by Response Surface Methodology and Its Antioxidant Activities
(1.Faculty of Food Science and Technology,Suzhou Polytechnic Institute of Agriculture,Suzhou 215008,Jiangsu,China;2.School of Food Science,Henan Institute of Science and Technology,Xinxiang 453003,Henan,China)
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投稿时间:2021-04-05    
中文摘要: 以花椒叶为原料,对花椒叶多酚提取工艺及其抗氧化活性进行研究。通过单因素试验分析料液比、提取温度、提取时间对花椒叶多酚提取率的影响,在单因素试验基础上,利用响应面法优化提取工艺。优化得到各因素最佳参数为料液比1∶56(g/mL)、提取温度81℃、提取时间107 min,验证试验所得实际多酚提取率为4.63%,与预测值接近。抗氧化活性研究结果表明花椒叶多酚对DPPH自由基、羟自由基、ABTS+自由基均有良好的清除能力。
Abstract:Polyphenols in Zanthoxylum bungeanum leaves were extracted.The extraction process and antioxidant activity of the polyphenols were studied.To be specific,the effects of material-to-liquid ratio,extraction temperature,and extraction time on the extraction rate of polyphenols were investigated.Basing on the results of the single factor experiments,the extraction process was optimized by response surface methodology.The yielded optimal extraction parameters were as follows:material-to-liquid ratio of 1∶56(g/mL),extraction temperature at 81℃,and extraction for 107 min.The actual extraction rate of polyphenols(4.63%)in the confirmatory test was close to the predicted value.Antioxidant research results showed that polyphenols from Zanthoxylum bungeanum leaf exhibited strong ability to scavenge DPPH free radicals,hydroxyl free radicals,and ABTS+free radicals.
文章编号:202206009     中图分类号:    文献标志码:
基金项目:河南省科技攻关项目(202102110060);江苏高校“青蓝工程”资助(苏教师函〔2020〕10号);苏州农业职业技术学院标志性成果(项目)培育基金项目(19BZ1002);江苏省高等学校大学生创新创业训练计划项目(202012808025Y)
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