Abstract:In order to realize efficient extraction and reveal the composition of flavonoids from chestnut shell，four factors（ethanol concentration，liquid-solid ratio，extraction temperature and extraction time）affecting the yield of flavonoids in chestnut shell were optimized by response surface methodology.Moreover，the composition of flavonoids in chestnut shell was analyzed by ultra-performance liquid chromatography-electrospray ionization-tandem mass spectrometry（UPLC-ESI-MS/MS）.The results demonstrated that the optimal extraction conditions were as follows：ethanol concentration of 70%，liquid-solid ratio of 17∶1（mL/g），extraction temperature of 65 ℃，and extraction time of 70 min.Under the optimized conditions，the yield of total flavonoids from chestnut shell reached 6.14%.The 6，7，8-trihydroxy-5-methoxyflavone in chestnut shell extracted under these conditions showed the highest content，followed by diosmetin.