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食品研究与开发:2022,43(5):1-7
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半胱氨酸对模拟体系中丙烯酰胺的抑制作用研究
(云南农业大学食品科学技术学院,云南 昆明 650201)
Inhibitory Effect of L-Cysteine on Acrylamide in A Model System
(College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,Yunnan,China)
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投稿时间:2021-03-11    
中文摘要: 以葡萄糖/天冬酰胺模拟体系为对象,研究褐变度及丙烯酰胺含量的变化规律,进一步探究半胱氨酸(L-cysteine,Cys)对模拟体系中丙烯酰胺的抑制作用。将葡萄糖与天冬酰胺溶液混合并在不同时间(0~40 min)和不同温度(160℃~220℃)下进行反应,通过测定模拟体系的褐变度及丙烯酰胺含量,分析时间和温度对模拟体系的影响;在模拟体系中加入Cys,测定葡萄糖、丙烯酰胺含量及褐变度,分析添加Cys对模拟体系的影响。结果表明,当温度达180℃及以上时,40 min内模拟体系的褐变度和丙烯酰胺含量均能达到峰值,随后降低;反应温度为160℃时,40 min内模拟体系的褐变度和丙烯酰胺含量随时间延长而升高;丙烯酰胺含量随Cys添加量增加而减少,且当添加量为100.0 mg时,丙烯酰胺的抑制率达到(93.8±0.2)%;在180℃模拟体系中添加50.0 mg Cys,其褐变度及丙烯酰胺含量升高趋势与对照组相比较缓慢,且峰值显著降低(P<0.05),对葡萄糖含量的变化趋势无明显影响。因此,Cys能显著降低葡萄糖/天冬酰胺模拟体系的褐变度及丙烯酰胺含量。
Abstract:The study examined the mechanism underlying the change in browning degree and acrylamide content in a glucose/asparagine model system.Additionally,the inhibitory effect of L-cysteine (Cys)on acrylamide in the model system was explored.The solutions of glucose and asparagine were mixed and reacted for different durations(0-40 min)and at different temperatures(160℃-220℃).Measuring the browning degree and acrylamide content of the model system enabled the analyses of the influence of time and temperature on the model system.After adding Cys to the model system,the effects on the content of glucose,acrylamide and browning degree were determined.When the temperature was 180℃ or above,the browning degree and acrylamide content of the system were the greatest and then decreased within 40 min.When the reaction temperature was 160℃,the browning degree and acrylamide content of the model system increased with time within 40 min.The content of acrylamide decreased with increasing Cys.The inhibition rate of acrylamide reached (93.8±0.2)% when 100.0 mg Cys was added.When 50.0 mg Cys was added to the model system at 180℃,the browning degree and acrylamide content increased more slowly than those of the control group,and the peak value was significantly reduced (P<0.05),with no obvious effect on the changing trend of glucose.Therefore,Cys can significantly reduce the browning degree and acrylamide content in the glucose/asparagine model system.
文章编号:202205001     中图分类号:    文献标志码:
基金项目:国家自然科学基金项目(31860474、32060573);云南省应用基础研究计划面上项目(2017FB029);云南省教育厅科学研究基金项目(2019J0117)
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