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投稿时间：2021-06-22
投稿时间：2021-06-22
中文摘要: 该试验旨在建立一种高效液相色谱法（high performance liquid chromatography，HPLC）检测蛋鸡饲喂白藜芦醇后获得的试验鸡蛋中白藜芦醇富集情况的方法。使用C18（4.6 mm×250 mm，5 μm）色谱柱，以乙酸+水（0.9 mL+750 mL）∶乙腈=65∶35（体积比）为流动相；流速 1.0 mL/min；检测波长 306 nm；柱温 30 ℃；进样量 10 μL。结果表明：白藜芦醇在线性范围1 μg/mL～10 μg/mL内标准品浓度与峰面积线性关系良好，标准曲线方程为y=72.777x，相关系数R2=0.999 7，平均加标回收率为86.72%～99.41%，RSD范围为0.5%～1.05%；使用该方法对鸡蛋蛋黄中白藜芦醇进行检测，结果显示，普通鸡蛋组蛋黄中不含白藜芦醇，添加1.5 g/kg白藜芦醇组和3 g/kg白藜芦醇组蛋黄中白藜芦醇含量分别为0.083 μg/g和0.371 μg/g。该方法精密度较高，出峰良好，与杂峰完全分离，适用于试验鸡蛋中的白藜芦醇的测定。
Abstract:The objective of this study was to establish a high performance liquid chromatography（HPLC）method to quantitate resveratrol enrichment in experimental eggs produced by laying hens whose diet was supplemented with resveratrol.A C18（4.6 mm×250 mm，5 μm）column was used with acetic acid+water（0.9 mL+750mL）∶acetonitrile=65∶35（volume ratio）as mobile phase.Flow rate was 1.0 mL/min.Detection wavelength was 306 nm，and column temperature was 30℃.Injection volume was 10 μL.A good linear relationship was observed between peak area and resveratrol standard concentrations，with a linear range of 1 μg/mL-10 μg/mL.The standard curve equation was y=72.777x，and the correlation coefficient was R2=0.999 7.Average recoveries were 86.72%-99.41%，and RSD range was 0.5%-1.05%.No resveratrol was detected in the yolks of the normal egg group.Resveratrol content in the yolks of the 1.5 g/kg and 3 g/kg resveratrol groups were 0.083 μg/g and 0.371 μg/g，respectively.The method was accurate，and suitable for quantitation of resveratrol in experimental eggs.
文章编号：202204025 中图分类号： 文献标志码：
基金项目:国家自然科学基金项目（31702223）；天津市企业科技特派员项目（19JCTPJC59500）；天津市科技重大专项与工程项目（18ZXBFNC00310）；天津市“131”创新型人才团队建设项目（20180318）
| 作者 | 单位 |
| 毋春楠，吴凤明，蔡锋隆，范真真，张宇强，毛帅强，于晓雪，李留安 | 天津农学院动物科学与动物医学学院天津市农业动物繁育与健康养殖重点实验室，天津 300392；天津市广源畜禽养殖有限公司，天津 301806 |
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