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投稿时间：2021-04-28
投稿时间：2021-04-28
中文摘要: 以冰岛刺参内脏团（含性腺、消化道、胃囊等）为原料，利用超声波辅助法提取冰岛刺参内脏团蛋白质，在单因素试验基础上，采用响应面分析法对蛋白质提取工艺进行优化，并对所得蛋白质进行氨基酸分析。最佳提取工艺为pH11.2，料液比1∶41（g/mL），超声温度57.6℃，超声时间38 min，超声功率80 W，在此条件下蛋白质提取率可达（82.87±0.12）%。氨基酸分析表明，冰岛刺参内脏团蛋白质富含必需氨基酸、鲜味氨基酸和药效氨基酸，分别占氨基酸总量的39.06%、40.89%和64.30%，营养价值较高，可以作为动物蛋白质的良好来源。
Abstract:The visceral mass （including gonads，digestive tract，and gastric pouches）of the sea cucumber Cucumaria frondosa was used as raw material to extract proteins through an ultrasonic assisted method.Based on a single factor test，the response surface analysis method was used to optimize protein extraction，and the protein obtained was subjected to amino acid analysis.The best extraction process was at pH11.2，material-to-liquid ratio 1∶41（g/mL），ultrasonic temperature 57.6 ℃，ultrasonic time 38 min，and ultrasonic power 80 W；under these conditions the protein extraction rate reached（82.87±0.12）%.Amino acid analysis showed that the Cucumaria frondosa protein from the visceral mass was rich in essential，umami，and pharmacodynamics amino acids，accounting for 39.06%，40.89%，and 64.30% of the total amino acids，respectively.The protein had high nutritional value and could be used as a good source of animal protein.
keywords: Cucumaria frondosa visceral mass protein ultrasonic assisted extraction response surface methodology amino acid analysis
文章编号：202204014 中图分类号： 文献标志码：
基金项目:山东省自然科学基金（ZR2020QC221）
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