Abstract:The effects of Leccinellum crocipodium （Letellier.）Watliag polysaccharides（LCP）on laxative and serum biochemical indices in mice were investigated in the present study.Sixty male BALB/c mice were randomly divided into five groups：blank group （treated with distilled water），fructooligosaccharide group（treated with 80 mg/kg），high-，medium-，and low-dose LCP groups（treated with 160，80，and 40 mg/kg LCP，respectively）.After continuous gavage administration for 30 d，fecal water content，fecal pH ，defecation time，small bowel motility，colon index，colon length，serum protein composition，serum lipid composition，and serum glucose content in each group of mice were measured.The results showed that continuous gavage administration of various doses of LCP did not affect the body weights of mice.However，LCP significantly increased fecal moisture content，ink advance rate ，colon index，and colon length of the tested mice（P＜0.05），and significantly reduced fecal pH and defecation time in the tested mice（P＜0.05）in a dose-dependent manner.Triglyceride ，total cholesterol，and low-density lipoprotein cholesterol contents in mice serum decreased following treatment with 160 mg/kg LCP.Therefore，LCP enhanced intestinal moisture and alleviated constipation，which could reduce fecal pH，and promote intestinal development and lipid metabolism in mice.