Abstract:The study was conducted to investigate the effect of mulberry leaf alkaloids（MLA）on glucose and lipid metabolism disorders in mice with a metabolic syndrome induced by a high fructose diet.An animal model of metabolic syndrome in high-fructose mice was established.Three doses of MLA[40 mg/（kg·d），80 mg/（kg·d）and 160 mg/（kg·d）]were administered.The biochemical indexes related to glucose and lipid metabolism were monitored，and the liver tissue structure was studied.Additionally，the effects of MLA on oxidative stress and inflammation in mice were investigated.The results showed that compared with the control group，the body weight of the model group increased significantly.The fasting blood glucose levels showed no significant difference，but glucose tolerance was significantly reduced，and the liver tissue structure was damaged.Serum triglyceride（TG），total cholesterol（TC），alanine aminotransferase（ALT），aspartate transaminase（AST），malondialdehyde（MDA），tumor necrosis factor α（TNF-α），interleukin-1β（IL-1β），and interleukin-6（IL-6）were significantly increased.The activity of superoxide dismutase（SOD）was significantly decreased.MLA intervention could in-hibit abnormal weight gain caused by a high fructose diet；improve glucose tolerance；reduce serum TC，TG，ALT，AST，MDA levels；increase serum SOD activity；improve the pathological damage to liver tissue；and reduce the levels of inflammatory factors TNF-α，IL-1β，and IL-6.These results confirmed that MLA could improve the glucose and lipid metabolism disorder induced by a high fructose diet in mice.The protective effect of MLA may be attributed to the improvement in antioxidant capacity and inhibition of inflammatory response.