Abstract:An ultrasonic-assisted ethanol extraction method was used to extract perilla flavone.Escherichia coli，Staphylococcus aureus，mucedine and Saccharomycetes were used as test organisms.A filter paper method and other research methods were used for the analysis.The antimicrobial activity of perilla flavone was investigated by measuring the inhibitory circle，minimum inhibitory concentration （MIC），minimum bactericidal concentration（MBC）and other indexes.The pH stability and thermal stability of perilla flavone were also studied.The results showed that the flavonoids obtained from perilla extract exhibited certain antibacterial effects against the four types of antimicrobials，with the strongest antibacterial activity against E.coli（MIC：0.625 mg/mL and MBC：1.3 mg/mL），followed by that against S.aureus （MIC：1.25 mg/mL and MBC：2.5 mg/mL），Saccharomycetes（MIC：2.41 mg/mL and MBC：4.8 mg/mL）and mucedine（MIC：2.5 mg/mL and MBC：5 mg/mL）.Under the MIC concentration of each bacteria，in the pH value of 3-7，the growth of the tested microorganisms was completely inhibited.Control temperatures less than 100℃showed no effect on the antibacterial ability of perilla flavone.However，when the temperature increased to 121 ℃ or higher，it negatively affected the antibacterial activity.