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投稿时间：2020-12-14
投稿时间：2020-12-14
中文摘要: 该研究以勃氏甜龙竹为研究对象，提取勃氏甜龙竹竹叶膳食纤维（Dendrocalamus brandisii leaves fiber，DBLF），测定其组成、理化特性，借助扫描电镜对其微观结构进行观察，并与市售微晶纤维素的理化性质进行比较。结果表明，DBLF 中含有可溶性膳食纤维（soluble dietary fiber，SDF）（10.07±0.01）%，不溶性膳食纤维（insoluble dietary fiber，IDF）（80.52±0.01）%，总膳食纤维（90.59±0.01）%。DBLF理化性质表明，持水力和水溶性指数分别为（6.06±0.09）g/g和 0.63%，对饱和脂肪酸、不饱和脂肪酸、葡萄糖吸附量分别为（2.91±0.04）、（1.26± 0.23）g/g、（15.69± 0.38）mmol/g。DBLF在中性条件下胆固醇的吸附量大于酸性条件[pH2，吸附量（5.19±0.22）mg/g；pH7，吸附量（8.45±0.46）mg/g]。葡萄糖延迟指数（glucose lag index，GRI）最大值出现在透析30 min（41.8%），并随透析时间的延长GRI逐渐减小。扫描电镜结果表明，DBLF颗粒表面粗糙，表面分布大小不一的孔洞和空穴，分布无规律。综上，与微晶纤维相比，DBLF具有较平衡的膳食纤维组成，较高的脂肪和葡萄糖吸附能力，较好的葡萄糖延迟指数，电镜结构多孔洞和空穴，呈蜂窝状结构。
Abstract:The extraction process of Dendrocalamus brandisii leaves fiber（DBLF）was optimized through single factor and orthogonal experiments.The nutritional composition and physical and chemical properties of DBLF were determined，and its microstructure was observed using a scanning electron microscope.Results determined that the dietary fiber in DBLF comprises soluble dietary fiber（10.07±0.01）%，insoluble dietary fiber（80.52±0.01）%and total dietary fibre（90.59±0.01）%.The physical and chemical properties of DBLF showed that the water holding capacity and water solubility index were（6.06 ± 0.09）g/g and 0.63%，respectively.The adsorption capacity for saturated fatty acid，unsaturated fatty acid，and glucose was（2.91±0.04），（1.26 ± 0.23）g/g and（15.69 ± 0.38）mmol/g，respectively.When pH2，the adsorption capacity of cholesterol was（5.19 ± 0.22）mg/g.When pH7，the adsorption rate of cholesterol was（8.45±0.46）mg/g.The maximum value of glucose lag index（GRI）appeared at 30 min of dialysis（41.8%）and decreased with the extension of dialysis.The structure of the scanning electron microscope showed that the surface of the DBLF particles was rough，with obvious pores and cavities on the surface.The pores were of different sizes，irregular distribution，loose and porous.In summary，compared with microcrystalline fiber，DBLF had a more balanced dietary fiber composition，higher fat absorption and glucose attachment capacity，and better glucose delay index.
keywords: Dendrocalamus brandisii bamboo leaves dietary fiber physicochemical property structure of the scanning electron microscopy（SEM）
文章编号：202122001 中图分类号： 文献标志码：
基金项目:国家自然科学基金项目（31860650）；云南省教育厅基金项目（2018Y132）
| Author Name | Affiliation |
| MA Si-yu，OUYANG Wu-le，LIU Xiao-he，HAN Ming-sheng，YANG Ya-jin，GUO Ai-wei | College of Life Science，Southwest Forestry University，Kunming 650224，Yunnan，China |
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