Abstract:ε-polylysine and chelate were combined to control the spoilage microorganisms in aftermarket mud carp （Cirrhinus molitorella）surimi.Nine strains of spoilage microorganisms were isolated from the mud carp surimi and identified using a microbiological method.Five dominant strains and three standard strains were selected for the screening of ε-polylysine synergy.According to the screening results，two ε-polylysine complex bio-preservatives were prepared，their bacteriostatic and antiseptic effect in chilled mud carp surim were studied.The results showed that ethylene diamine tetraacetic acid disodium salt （EDTA-2Na），glycine，L-alanine，and L-malic acid had a strong synergistic effect with ε -polylysine.The complex bio-preservative showed a good inhibitory effect on the test strains.The total number of colonies and the content of volatile base nitrogen in mud carp surimi were significantly reduced by the complex bio-preservatives compared with those in the control group.